Quantitative Flow Cytometry Measurements
This project aims to improve flow cytometry measurements by developing quality control methods, calibration standards, and assays for disease biomarkers.
This grant is no longer accepting proposals
NRC Research Associateship Programs has archived this opportunity.
Funder: NRC Research Associateship Programs
Due Dates: February 1, 2025 | May 1, 2025 | August 1, 2025 | November 1, 2025
Funding Amounts: Base stipend approximately $82,764/year with $3,000 travel allowance; typical appointment duration 2 years.
Summary: Supports postdoctoral research to develop quality control methods, calibration standards, and assays for quantitative flow cytometry measurements of disease biomarkers.
Key Information: Open to U.S. citizens holding a doctoral degree; applications require prior contact with research adviser; NIST participates in February and August review cycles only.
Description
This fellowship opportunity at the National Institute of Standards and Technology (NIST) focuses on advancing quantitative flow cytometry measurements, which are critical in clinical laboratories for assessing cell counts and disease biomarkers on lymphocytes from blood, lymph nodes, or bone marrow. Accurate quantification of these biomarkers informs cell properties such as lineage, clonality, differentiation stage, and activation status, impacting disease diagnosis, prognosis, and therapy decisions.
Currently, there is no widely accepted quality control method or calibration standard for multicolor flow cytometry, including instrument linearity, dynamic range, and sensitivity. This gap limits progress in medical research and healthcare delivery. The project aims to develop measurement methodologies, protocols, and calibration standards to improve the reliability and performance of quantitative flow cytometry. Additionally, it includes developing assays to study intracellular signal transduction pathways and to profile drug and therapy efficacy.
Key research areas include fluorescence calibration, biological controls, antibodies bound per cell, intracellular applications, and equivalent reference fluorophores (ERF).
